Poresyn Solutions Hyper Link CPG(HL-CPG)
2'-O-Methyl CPG Co-Polymer Manufacturer
Poresyn Solutions‘ 2’-O-Methyl Modified CPG co-polymer offer:
Up to 6× higher nucleotide loading capacity, enabling more efficient industrial-scale synthesis of 2’-OMe oligonucleotides such as ASO and siRNA.
Small MOQ
We support small MOQs, starting from 10 g, for the Universe linker type.
Tailored Solutions
No matter what kind of linker you need, we can fulfill unique requests.
Free Sample For Test
Providing small sample of our HL-CPG with Unylinker for testing without charge
Free Delivery
Free air shipping, typically, the things arrive within seven days.
2'-O-Methyl CPG (Co-Polymer)
Main Advantages of our Co-Polymer Coated CPG
- Ultra-High Loading: Up to six times greater than corresponding functionalized CPGs. For instance, a loading of up to 120 μmol/g is possible with our 2000 Å 2'-OMe Modified HL-CPG.
- High Purity: Crude oligo purity of more than 80% is made possible by our co-polymer coated solid support's inertness to chemical reagents and solvents during synthesis and post-treatment.
- High Output: Depending on the length and complexity of the oligos, our co-polymer coated CPG can raise A260 OD values by more than 6× than corresponding functionalized CPG does.
- No Silica Contamination: Our solid support greatly lowers downstream purification costs by preventing silica contamination due to the special PS+CPG crosslinked structure.
- No Swelling: Our co-polymer coated CPG does not swell during synthesis, in contrast to conventional polystyrene resins, guaranteeing reliable and secure production.
2'-OMe Modified HL-CPG with High-Loading for Complex gRNA Synthesis
Depending on the complexity of the gRNA being produced, our 2′-OMe modified HL-CPG yields 3–6× greater nucleotide loading than corresponding functionalized CPGs. Even when steric hindrance occurs during long-chain synthesis, our effective loading is still more than three times that of traditional CPGs because of our patented PS+CPG crosslinked structure.
In addition to maximizing oligo production, its special co-polymer coated architecture guarantees stability and homogeneity throughout the synthesis process. Our HL-CPG is the best option for producing complicated oligonucleotides on an industrial scale since it offers a dependable and repeatable solution for long gRNAs longer than 100 nt.
Significantly Lowerdown COG (Cost of Goods)
We achieve excellent oligo yields because of our co-polymer coated CPG’s ultra-high loading capacity, consistent physical structure, batch-to-batch consistency, improved chemical reactivity and lower impurity levels. Overall, compared to traditional functionalized CPGs, the A260 OD values might be up to 3–6× greater.
Moreover, the better purity of our product, absence of silica leaching, and reduction of CPG fragment-related pipeline blockage can lower downstream purifying costs by approximately 30%. In conclusion, compared to traditional CPGs, utilizing our HL-CPG (also called co-polymer coated CPG) greatly reduces the total synthesis cost.
What is a 2′-O-Methyl (2′-OMe) modification?
The hydroxyl group (-OH) at the 2′ position of a natural RNA ribose is extremely reactive and readily broken by nucleases. A 2′-O-Me modification creates a significantly more stable structure by changing the 2′ position to a methoxy group (–O–CH₃). This prevents the RNA from being misinterpreted as a viral signal by greatly increasing nuclease resistance and decreasing detection by immune receptors like TLR7/8.
Additionally, the change improves binding affinity to the target mRNA by strengthening base-pairing interactions. In solid-phase synthesis, 2′-O-Me provides better chemical stability and minimizes intra- or inter-strand side reactions, leading to more regulated and effective coupling, particularly for longer RNA sequences (>60–120 nt).
Co-Polymer Coated-CPG
Our 2'-O-Methyl Modified CPG (Co-Polymer)
Following are the principal monomer types linked to our co-polymer coated CPG solid support for RNA synthesis.
OUR PRODUCT
Superior Advantages of our HL-CPG (Co-Polymer Coated CPG Solid Support)
By utilizing our HL-CPG, users typically achieve a 30–70% reduction in cost of goods (COG) compared with conventional functionalized CPG, thanks to its high loading capacity, superior purity, and overall process efficiency
High Loading
3–6× higher loading than traditional CPG supports — e.g., our 2000Å HL-CPG achieves 160 μmol/g
High Purity
Delivers >80% oligo purity, owing to strong chemical resistance to reagents and solvents
No Swelling
Unlike traditional polystyrene supports, our HL-CPG remains stable and does not swell under reaction or heating conditions, ensuring consistent synthesis performance
High surface reactivity
The ps layer provides high surface reactivity, resulting in outstanding synthesis performance even when no LCAA is coupled.
No Silica Contamination
No glass particles are generated during ammonolysis, thanks to the PS surface crosslinked with the CPG core structure, ensuring cleaner synthesis and easier purification.
Fully Compatible Setup
Fully compatible with existing CPG-based synthesis setups — no equipment modification required
Why Our 2′-OMe CPG Is the Best Choice for Synthesizing gRNA
One of the most popular stabilizing techniques in CRISPR gRNA creation is 2′-O-Methyl (2′-OMe) modification. However, synthesizing lengthy gRNA (70–120 nt) is still technically difficult because of the bulky structure and greater reactivity of 2′-OMe monomers.
We specifically designed our HL-CPG (co-polymer coated CPG) to get over these restrictions.
Customers can achieve a much larger synthesis scale with ultra-high loading capacity (e.g., our 2000Å 2′-OMe modified HL-CPG reaches up to 120 μmol/g). Furthermore, HL-CPG provides better coupling efficiency, which is particularly important for the sterically demanding 2′-OMe monomers. Our extremely homogeneous pore/particle structure and the increased chemical activity offered by the PS-coating surface are the sources of this performance advantage.
30% improve in purity
Because of this, HL-CPG regularly yields crude purity that is over 30% higher than that of traditional CPG. Our FLP output remains at 3–6× greater A260 OD when compared to normal CPG, even when real-world obstacles like RNA structural complexity, difficult process conditions, and significant steric hindrance are taken into account.
For example , typically, our 2000Å HL-CPG can reach OD values above 5000 for long gRNA (>100 nt), allowing for actual industrial-scale RNA manufacture, the huge increase of OD value post significantly value to oligonucleotide therapeutics industry.
What is the maximum RNA length your 2000Å co-polymer coated CPG can support?
Theoretically, our 2000Å HL-CPG can support RNA synthesis up to 300 nt.
For 100–200 nt RNA, we are confident that our solid support is currently the most optimal option available in the market.
Does your product swell during synthesis? Do we need to consider packing density?
No. Our co-polymer coated CPG does not swell, so no packing-density adjustment is required.
Is the loading of your 2000Å HL-CPG adjustable?
Yes. The loading capacity of our standard 2000Å HL-CPG ranges from 80–180 μmol/g.
We typically recommend starting at ~80 μmol/g. If your synthesis efficiency is high, you may increase the loading to determine the optimal level for your process.
Can your co-polymer coated CPG be integrated with a cell delivery system?
Actually, we can install cell delivery much more easily and effectively than with equivalent functionalized CPG because the ps layer is covered with the core structure of controlled pore glass.
Do you sell standalone 2′-O-Methyl (2′-O-Me) modified nucleoside/nucleotide monomers?
Yes, we can also supply 2′-O-Methyl modified nucleoside/nucleotide monomers alone, without HL-CPG.
Can you add LCAA on your HL-CPG?
certainly we can increase our CPG’s chemical reactivity by adding LCAA. Please be aware, nevertheless, that there won’t be any issues without adding LCAA because our HL-CPG is already covered with a high reactivite polystyrene surface that offers high nucleic efficacy synthesis.
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2'-O-Methyl CPG
Related Product
Custom modifications of our co-polymer coated controlled pore glass, such as DNA/RNA modified HL-CPG, 2′-O-Methyl modified, reverse functionalized, LNA modified, 2′-F modified, and others, are typically available upon request.
RNA Modified CPG Co-Polymer
DNA Modified CPG Co-Polymer
Reverse Functionalized CPG Co-Polymer
LNA Modified CPG Co-Polymer
2'-F Modified CPG Co-Polymer
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Test the power of our Super Solid Support - HL-CPG
Would you like to test our next-generation co-polymer coated CPG? Get in touch with us right now; we offer a free sample of our HL-CPG with unylinker for rapid testing.