Poresyn Solutions Hyper Link LNA Modified CPG
we offer super high loading LNA modified CPG co-polymer for medium to long chain (20-300nt) ASO, SiRNA.
3-6x higher loading and 30% improve in purity level than traditional functionalized CPG.
Small MOQ
We support small MOQs, starting from 10 g, for the Universe linker type.
Tailored Solutions
No matter what kind of linker you need, we can fulfill unique requests.
Free Sample For Test
Providing small sample of our HL-CPG with Unylinker for testing without charge
Free Delivery
Free air shipping, typically, the things arrive within seven days.
LNA Modified CPG (Co-Polymer)
Why Using Our LNA Modified CPG
- High Loading Capacity: Offers 3–6× higher loading compared with traditional LNA-modified CPG solid supports, enabling greater synthesis output per gram.
- High UV Purity: Performance Demonstrates excellent optical purity, with A260 OD values reaching the thousands level, indicating highly efficient synthesis and minimal impurities.
- Outstanding Stability of LNA and Linker: Ensures strong chemical stability of both the LNA starting nucleotide and the linker during synthesis cycles, minimizing premature cleavage and side reactions.
- GMP-Scale Production Capability: Supports GMP-grade manufacturing, with scalable production capacity, full documentation, and batch traceability suitable for clinical development and commercial applications.
GMP Production Cost Saving
Our HL-CPG solid support provides a loading capacity that is 3x-6x higher than that of corresponding functionalized CPG, along with increased synthesis efficiency. This means customers can achieve higher oligo yields using the same synthesis equipment and facilities, thereby making GMP-scale oligonucleotide production a reality.
Lower Down Purification Cost
Furthermore, by using our co-polymer coated CPG solid support the crude purity of oligonucleotides is significantly higher, resulting in huge cost savings in subsquent purifying process. Purification costs can be saved by at least 30% when compared to traditional CPGs, which is a significant cost savings for biotech companies.
Solving the Long-Chain RNA Synthesis Challenge
In today’s biopharmaceutical landscape, the synthesis of long oligonucleotides—especially RNA sequences above 100 nt—remains extremely difficult. Compared with DNA, RNA features a more complex structure and therefore places much higher demands on the solid support material used during synthesis.
Poresyn Solutions' 2000A CPG co-polymer
At Poresyn Solution, our 2000 Å co-polymer coated CPG has been proven to successfully synthesize 100–200 nt RNA with high output, making it the most practical and scalable solution for industrial-grade production of long RNA therapeutics. This exceptional performance is enabled by our unique polystyrene + CPG cross-linked architecture, which delivers ultra-high loading and high crude purity without sacrificing long-chain synthesis capability.
High Output
As a result, the A260 OD value of RNA synthesized on our support is dozens of times higher than that of traditional CPG. While conventional CPG typically yields only tens to a few hundred OD, our HL-CPG routinely reaches 3,000–5,000 OD, offering biopharmaceutical companies a massive quantitative advantage for long-oligo manufacturing.
In summary, our 2000 Å HL-CPG currently represents the most effective and scalable solution for industrial-scale long-chain RNA synthesis.
What is LNA Modified CPG
LNA-modified CPG refers to a CPG solid support on which an LNA (Locked Nucleic Acid)–modified nucleoside is pre-attached as the first nucleoside for oligonucleotide synthesis.
LNA monomers are significantly more expensive than regular nucleotides, typically costing several times more due to their complex chemical structure.
The use of LNA brings major advantages, including:
Higher binding affinity to the target sequence
Greater structural stability of the oligonucleotide
Strongly enhanced resistance to nuclease degradation
Because of these benefits, LNA-modified CPG is commonly used for the synthesis of high-affinity nucleic acid therapeutics and detection oligos, such as ASO, siRNA, diagnostic probes, and other LNA-containing sequences.
Co-Polymer Coated CPG
Poresyn Solutions' LNA Modified Controlled Pore Glass - Co-Polymer
LNA-modified CPG (co-polymer) ： co-polymer coated CPG solid support on which an LNA (Locked Nucleic Acid) monomer or LNA-modified nucleoside is installed as the linker.
OUR PRODUCT
Superior Advantages of our HL-CPG (Co-Polymer Coated CPG Solid Support)
By utilizing Poresyn Solutions HL-CPG, users typically achieve a 30–70% reduction in cost of goods (COG) compared with conventional functionalized CPG, thanks to its high loading capacity, superior purity, and overall process efficiency
High Loading
3–6× higher loading than traditional CPG supports — e.g., our 2000Å HL-CPG achieves 160 μmol/g
High Purity
Delivers >80% oligo purity, owing to strong chemical resistance to reagents and solvents
No Swelling
Unlike traditional polystyrene supports, our HL-CPG remains stable and does not swell under reaction or heating conditions, ensuring consistent synthesis performance
High surface reactivity
The ps layer provides high surface reactivity, resulting in outstanding synthesis performance even when no LCAA is coupled.
No Silica Contamination
No glass particles are generated during ammonolysis, thanks to the PS surface crosslinked with the CPG core structure, ensuring cleaner synthesis and easier purification.
Fully Compatible Setup
Fully compatible with existing CPG-based synthesis setups — no equipment modification required
DNA Modified CPG Co-Polymer
HL-CPG — Superior PS + CPG Cross-Link Architecture
Our patented co-polymer coating technology represents a major advancement beyond traditional bare CPG supports.
Instead of using untreated CPG particles, we apply an ultra-thin polystyrene-based resin coating directly onto the CPG surface, with a thickness of typically less than 3 nm.
PS+CPG Cross-linked Structure
This coating is firmly cross-linked to the CPG matrix, delivering several key performance benefits:
- Provides high chemical reactivity for efficient oligonucleotide synthesis
- Shields the CPG surface from aggressive reagents and solvents during synthesis and post-treatment
- Prevents silica dissolution, ensuring no silicon contamination in the final crude product
High Output Performance
Thanks to this innovative structure, HL-CPG combines the best of both worlds:
- The ultra-high loading capacity and reactivity of resin-based solid supports
- The long-chain synthesis capability of traditional CPG, enabling reliable production of long and complex oligonucleotides
Why use LNA-modified CPG?
Using LNA-modified CPG is useful because LNA considerably boosts the binding affinity of oligonucleotides.
Although LNA is somewhat expensive, many biotech businesses still require the synthesis of LNA-containing oligonucleotides. For example, when creating ASO gapmers (DNA in the core area with LNA at both ends), LNA is necessary for achieving high-affinity oligonucleotides, and the synthesis often starts with LNA monomers.
Typical applications include antisense oligonucleotides (ASO), siRNA, molecular probes, diagnostic tests, PCR primers, and miRNA detection, where better affinity and improved stability are crucial for performance.
Structure of LNA-modified CPG(Co-Polymer)
LNA-modified HL-CPG normally consists of three components:
1. Our co-polymer covered CPG base structure, commonly with pore diameters of 500Å, 1000Å, or more.
2. A silanized functional group, which creates a reactive layer on the surface of the HL-CPG.
3. An associated LNA initial nucleoside (LNA-A, LNA-C, LNA-G, or LNA-T/U).
In essence: The first nucleoside, which is an LNA, is fastened onto our HL-CPG, functioning as the starting point of the strong support.
Thus, it is referred described as: LNA-modified CPG = LNA linker + functionalized CPG.
Poresyn Solutions' LNA CPG
Compared to typical CPG solid supports, our HL-CPG permits easier inclusion of various specially modified monomers, and offers increased loading capacity and purity, making it appropriate for industrial-scale GMP manufacturing.
Which types of LNA starter nucleotides with your hyper link CPG can be offered?
We offer multiple LNA-modified starter nucleotides on HL-CPG, including:
LNA-A (Bz)
LNA-C (Bz)
LNA-G (dmf)
LNA-T (U)
Can your LNA-modified HL-CPG support long-chain oligonucleotide synthesis (e.g., >100 nt)?
Yes. Our HL-CPG combines a high-loading polystyrene coating with a CPG core, enabling both high reactivity and long-chain synthesis capability.
Even with LNA as the first nucleotide, HL-CPG maintains excellent coupling efficiency and high crude purity and high A260 OD yield.
What specific advantages does HL-CPG offer over traditional LNA-modified CPG?
Key performance benefits include:
3–6× higher loading capacity
Higher crude UV purity, reducing downstream purification costs
No silica contamination and no swelling
Fully compatible with existing CPG synthesis equipment and SOPs (drop-in replacement)
Does your LNA-modified CPG ensure strong stability during synthesis and deprotection process?
Yes. The LNA linker chemistry and our co-polymer coating CPG structure have been optimized to ensure high stability under synthesis reagents, high-temperature post-treatment, and acidic deprotection conditions.
This prevents LNA cleavage or premature detachment, and result in the consistently high final oligonucleotide yields.
Can you add LCAA on your HL-CPG?
certainly we can increase our CPG’s chemical reactivity by adding LCAA. Please be aware, nevertheless, that there won’t be any issues without adding LCAA because our HL-CPG is already covered with a high reactivite polystyrene surface that offers high nucleic efficacy synthesis.
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Related Product
Custom modifications of our co-polymer coated controlled pore glass, such as DNA/RNA modified HL-CPG, 2′-O-Methyl modified, reverse functionalized, LNA modified, 2′-F modified, and others, are typically available upon request.
RNA Modified CPG Co-Polymer
DNA Modified CPG Co-Polymer
2'-O-Methl Modified CPG Co-Polymer
Reverse CPG Co-Polymer
2'-F Modified CPG Co-Polymer
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Test the power of our Super Solid Support - HL-CPG
Would you like to test our next-generation co-polymer coated CPG? Get in touch with us right now; we offer a free sample of our HL-CPG with unylinker for rapid testing.