2′-Fluoro-U CPG Co-Polymer Coated

What is 2′-Fluoro-U CPG?

2′-Fluoro-U CPG is a premium modified solid support matrix used in automated solid-phase oligonucleotide synthesis to precisely introduce a 2′-fluoro-2′-deoxyuridine analogue at the 3′ terminus of a sequence. Since the uracil (U) nucleobase does not possess an exocyclic amine group, it requires no base protection, ensuring a clean and streamlined chemical profile during coupling loops. By replacing the 2′-hydroxyl group of the ribose ring with an electronegative fluorine atom, this support locks the sugar conformation into a highly stable RNA-like C3′-endo structure. This pre-organization is critical for developers engineering customized siRNA, antisense oligonucleotides (ASOs), and aptamers that require maximum thermodynamic duplex stability (ΔTm ) and strong metabolic resilience against enzymatic degradation by cellular nucleases.

1. Product Description

2′-Fluoro-U Co-Polymer Coated CPG

Product Overview

2′-Fluoro-U Co-Polymer Coated CPG is a professional-grade, high-capacity solid support matrix specifically engineered for the automated chemical synthesis of modified oligonucleotides. This specialized support provides a reliable chemical framework for the clean and efficient incorporation of a 2′-fluoro modified U nucleobase at the 3′ end of an oligonucleotide chain.

Because uracil lacks exocyclic amino functions, this solid support eliminates the need for base-specific protecting groups, resulting in excellent chemical purity and zero base-modification side reactions during solid-phase automated synthesis cycles. Substituting the natural 2′-OH group with a fluorine atom biases the ribose sugar conformation toward a rigid C3′-endo pucker. This pre-organization perfectly mimics RNA structural characteristics, significantly increasing the thermal melting temperature (ΔTm ) of resulting duplexes with complementary RNA target strands and rendering the 3′ terminus highly resistant to cellular exonucleases. It is a fundamental solid support for modern RNA interference (RNAi) therapeutics and gapmer engineering.

Key Technical Features & Guidelines

• Targeted 3′-End 2′-Fluoro U Modification: Engineered specifically for the flawless solid-phase placement of a 2′-fluoro modified U nucleobase at the 3′ terminus of the oligonucleotide chain.

• Streamlined Uracil Architecture: The absence of base protection simplifies final cleavage and deprotection parameters, fitting seamlessly into standard downstream processing lines.

• Enhanced Duplex Binding Affinity: Stabilizes the A-form helical structure upon hybridization, ensuring exceptional thermodynamic affinity and binding kinetics toward complementary mRNA or microRNA targets.

• Advanced Co-Polymer Coating Technology: Our proprietary crosslinked co-polymer surface matrix prevents matrix bleeding and structural swelling under high-pressure automated fluidic runs, delivering steady column pressure and uniform contact time.

• Industrial Pore Size Infrastructure: Available in a versatile range of pore sizes—500 Å, 1000 Å, 2000 Å, and 3000 Å—to seamlessly accommodate high-throughput diagnostic screening, long-chain custom aptamers, and mass-loaded commercial therapeutic manufacturing.

Applications

• RNA Interference (RNAi) & ASOs: Introduction of 2′-fluoro stabilization at the 3′ end to prolong in vivo serum half-life and maximize gene-silencing potency.

• Thermodynamically Tuned Probes: Development of dual-labeled diagnostic assays, molecular beacons, and specific primers where fine-tuning the hybrid $T_m$ is critical.

• Nuclease-Resistant Aptamers: Production of highly stable structural aptamers capable of resisting enzymatic breakdown in physiological fluids.

Synthesis Optimization Note (Hyper-link CPG Benefits)

Poresyn Technologies’ 2′-Fluoro-U CPG product line leverages our proprietary co-polymer coated matrix technology (Hyper-link CPG), which is available for all kinds of tailored linkers. This structural integration unlocks measurable benefits for industrial scale-up:

• 3× Higher Output & Lower COG: Our Hyper-link CPG delivers up to a 3× higher loading capacity compared to traditional corresponding un-coated supports, allowing laboratories to drastically lower their overall Cost of Goods (COG).

• ~30% Lower Purification Costs: Delivers exceptional Full-Length Product (FLP) purity straight out of the synthesis column, optimizing downstream chromatographic workflows.

• Reliable Long-Oligo Synthesis: Engineered to sustain high coupling efficiency for complex or extended sequences (100–300 nt) with zero structural collapse.

We supply this product as a standalone, high-performance solid support matrix in multiple bulk configurations to meet your specific research and commercial production scales.

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