2′-OMe rA(Bz) CPG – Co-Polymer Coated

What is 2′-OMe rA(Bz)?

2′-OMe rA(Bz) is a commonly used building block in RNA oligonucleotide chemical synthesis. The abbreviation represents 2′-O-methyl protected ribo-adenosine with an N6-benzoyl protecting group.

• 2′-OMe (2′-O-methyl) indicates that the ribose sugar contains a methyl modification at the 2′-hydroxyl position. This modification is widely used in therapeutic oligonucleotides to enhance nuclease resistance, molecular stability, and binding affinity.

• rA (ribo-adenosine) refers to the RNA nucleoside containing adenine as the nucleobase.

• Bz (benzoyl) represents the benzoyl protecting group attached to the N6 amino position of adenine during synthesis.

2′-O-methyl modified nucleotides are widely used in modern RNA therapeutics, including siRNA, antisense oligonucleotides (ASO), and CRISPR RNA components, due to their improved stability and biological compatibility.

Role of the Bz Protecting Group

During oligonucleotide synthesis, the N6 amino group of adenine is chemically reactive and may participate in unwanted side reactions during phosphoramidite coupling.

To prevent these reactions, the adenine base is protected with a benzoyl (Bz) group at the N6 position. This protection ensures efficient chain elongation and high synthesis fidelity.

After completion of the RNA chain assembly, the protecting group is removed during the standard cleavage and deprotection process, typically using aqueous ammonia or AMA solution, restoring the native 2′-O-methyl adenosine structure.

Applications of 2′-OMe rA(Bz)-CPG

2′-OMe rA(Bz)-CPG is used as the solid support for initiating oligonucleotide synthesis when the 3′-terminal nucleotide is 2′-O-methyl adenosine.

Typical applications include:

• siRNA synthesis – used when the 3′ terminal nucleotide of the RNA strand is 2′-OMe A

• Antisense oligonucleotides (ASO) – including gapmer or fully modified RNA designs

• CRISPR gRNA / sgRNA synthesis – for chemically synthesized RNA fragments

• Modified RNA oligonucleotides used in therapeutic and research applications

Advantages of Co-Polymer Coated 2′-OMe rA(Bz)-CPG

Poresyn Solutions’ co-polymer coated CPG solid support is designed to enhance the performance of RNA oligonucleotide synthesis, particularly for industrial and GMP-scale production.

Ultra-High Loading Capacity

The co-polymer coated structure enables 3–6× higher loading capacity compared with traditional functionalized CPG solid supports, significantly increasing oligonucleotide output per synthesis batch.

High Crude RNA Purity

The chemically resistant co-polymer layer provides excellent stability against acidic, basic, and organic reagents, resulting in higher crude RNA purity and reduced purification workload.

Rigid Non-Swelling Structure

Unlike polystyrene-based resins, CPG maintains a rigid and non-swelling structure during coupling, oxidation/sulfurization, and deprotection processes, ensuring stable synthesis performance.

No Silica Particle Contamination

The co-polymer coated surface prevents silica particle shedding during cleavage and deprotection, minimizing column clogging and backpressure during large-scale oligonucleotide synthesis.

More Resources

rC(Ac) 2’OMe Co-Polymer Coated CPG （controlled pore glass) solid support

rC(Bz) 2’OMe Co-Polymer Coated CPG （controlled pore glass) solid support

rG(ibu) 2’OMe Co-Polymer Coated CPG （controlled pore glass) solid support

rG(dmf) 2’OMe Co-Polymer Coated CPG （controlled pore glass) solid support

rU 2’OMe Co-Polymer Coated CPG （controlled pore glass) solid support