Product Overview
3′-DMT-dC(Bz)-Suc-CPG Co-Polymer Coated is a highly specialized, premium solid support matrix engineered for automated “reverse” (5′ to 3′ direction) oligonucleotide synthesis. While conventional chemical synthesis of DNA proceeds in a 3′ to 5′ direction due to standard 3′-O-phosphoramidite building blocks, our reverse synthesis matrix opens up critical structural possibilities for therapeutic nucleic acid design.
By immobilizing a 5′-linked deoxycytidine (dC) bearing N^4-benzoyl (Bz) protection onto the support, chain elongation is directed to proceed from the 5′ to the 3′ end. This strategic inversion allows for the convenient modification of only the linkage at the 3′ terminus, generating protective 3′–3′ terminal internucleotidic caps. This minimal modification results in effective exonuclease resistance—specifically against 3′-exonucleases, which represent the most significant enzymatic degradation route—with virtually zero disruption to natural hybridization or toxicity concerns.
Key Technical Features & Guidelines
Chemical Architecture: 5′-O-DMT-dC(Bz)-3′-succinoyl-LCAA-CPG. This protection strategy is fully compatible with standard automated DNA and RNA synthetic chemistry.
Extended Detritylation: Please note that 3′-DMT groups detritylate more slowly than standard 5′-DMT groups. It is highly recommended to increase the detritylation (deblock) time by 50% during the synthesis cycle.
Spectral Properties: Extinction Coefficient at 260 nm is 6200 (measured in water for the cleaved and deprotected nucleoside).
Advanced Co-Polymer Coating: Our proprietary crosslinked co-polymer matrix eliminates silica contamination and prevents structural swelling, ensuring consistent column pressure and steady fluidic flow rates.
Flexible Pore & Loading Dynamics: Available in 500 Å, 1000 Å, and 2000 Å pore sizes to seamlessly accommodate both high-throughput diagnostic arrays and heavily mass-loaded therapeutic scalability.
Applications
Nuclease-Resistant Therapeutics: Synthesis of antisense oligonucleotides (ASOs), siRNA, and aptamers with inverted 3′–3′ terminal linkages to significantly prolong in vivo half-life.
Structural Biology Research: Construction of directional inversion loops, alternating 3′–3′ and 5′–5′ hybridized motifs, and stabilized secondary structures.
Advanced Cell Delivery Conjugation: Functionalized synthesis lines incorporating cell-targeting modifications at specific structural termini.
💡 Synthesis Optimization Note (Hyper-link CPG Benefits)
Poresynsolutions‘ Reverse dC(Bz) CPG product line leverages our proprietary co-polymer coated matrix technology (Hyper-link CPG), which is available for all kinds of tailored linkers. This structural integration unlocks measurable benefits for industrial scale-up:
3× Higher Output & Lower COG: Our Hyper-link CPG delivers up to a 3× higher loading capacity compared to traditional corresponding un-coated supports, allowing laboratories to drastically lower their overall Cost of Goods (COG).
~30% Lower Purification Costs: Delivers exceptional Full-Length Product (FLP) purity straight out of the synthesis column, optimizing downstream chromatographic workflows.
Reliable Long-Oligo Synthesis: Engineered to sustain high coupling efficiency for complex or extended sequences (100–300 nt) with zero structural collapse.
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