dC(Ac) CPG Co-Polymer Coated

dC(Ac) and dC(Ac)-CPG for Oligonucleotide Synthesis

1. What is dC(Ac)?

dC(Ac) is a protected form of deoxycytidine used in DNA oligonucleotide synthesis, where the cytosine base is protected with an acetyl (Ac) group at the exocyclic amino position. The Ac group prevents unwanted side reactions, improves coupling efficiency, and ensures high fidelity during chain elongation.

2. Applications of dC(Ac)-CPG

dC(Ac)-CPG solid supports are widely used in the synthesis of therapeutic oligonucleotides, such as antisense oligonucleotides (ASOs), siRNAs, and guide RNAs (gRNAs) for CRISPR applications. Their incorporation at the 3′ end of oligonucleotides facilitates efficient synthesis of long-chain sequences.

3. Advantages of dC(Ac)-CPG in large-scale production

The use of dC(Ac) is particularly suitable for high-volume oligonucleotide synthesis due to several factors: low raw material cost, high coupling efficiency, and minimal steric hindrance during the synthesis process. This allows for effective production of long oligos with reduced cycle times and improved overall yield.

4. Benefits of co-polymer coated dC(Ac)-CPG in GMP-scale industrial oligo production

• Ultra-high loading capacity: 3–6× higher than traditional CPGs, significantly increasing oligonucleotide output. For example, our 2000 Å HL-CPG provides 160 μmol/g, far exceeding conventional functionalized CPGs.
• High crude oligo purity: >80% due to the solid support’s exceptional chemical and solvent resistance, reducing downstream purification requirements.
• Stable physical structure: No swelling occurs during synthesis or heating, unlike traditional polystyrene-based resins.
• No silica contamination: No CPG fragments are released during deprotection, preventing pipeline clogging, minimizing backpressure, and ensuring safe synthesis of long-chain oligonucleotides.