dC(Bz)-CPG Co-Polymer Coated

What is dC(Bz)?

dC(Bz) refers to N4-Benzoyl-2′-deoxycytidine, commonly called N4-benzoyl-protected deoxycytidine.

• dC = 2′-deoxycytidine (one of the four DNA nucleobases)
• (Bz) = Benzoyl, a base-protecting group

Why is Bz protection needed for dC?

Cytosine (C) has a reactive exocyclic amino group (N4-NH₂), which, if left unprotected, can cause:

• Increased side reactions
• Reduced coupling efficiency

Using Benzoyl (Bz) protection safeguards the N4-amino group, allowing DNA synthesis to proceed more stably, with stronger acid and oxidation resistance.

Applications of dC(Bz)-CPG

dC(Bz)-CPG is most commonly used to synthesize DNA oligonucleotides with a 3′-end cytosine. Typical applications include:

• PCR / qPCR primers (30–60 nt)
• DNA probes (20–40 nt)
• Antisense oligonucleotides (ASO, 15–25 nt)
• gRNA template DNA and other gene-editing related DNA

It is particularly recommended when the DNA sequence is >50 nt, or when dC(Ac) fails or shows instability. For short oligos (<20 nt), dC(Bz)-CPG is rarely required.

Why use Poresyn Solutions’ co-polymer coated CPG?

1. 3–6× higher loading than traditional CPGs, boosting oligo output for GMP-scale industrial production. For example, our 2000 Å HL-CPG has a loading capacity of 160 – 180μmol/g, far exceeding conventional functionalized CPGs.
2. >80% oligo purity, thanks to the solid support’s strong resistance to chemical reagents and solvents.
3. No swelling during synthesis or heating, unlike traditional polystyrene-based resins.
4. No silica contamination—no CPG fragments are generated during deprotection, eliminating pipeline clogging, reducing backpressure, and minimizing safety risks in long-chain oligo synthesis.

5. The reactive polystyrene coating on our solid support offers excellent chemical reactivity, enabling efficient and versatile conjugation with a wide range of functionalities, including:

   • Standard nucleotides, such as A, C, G, and T

   • Modified monomers, including 2′-O-Methyl, LNA, and 2′-F modified nucleotides

   • GalNAc ligands, universal linkers, and other specialized linkers

Our co-polymer coated CPG has been optimized for the latest therapeutic oligonucleotide classes, including LNA, delivery-enhanced lipid conjugates, siRNA, and Spiegelmers. The solid support provides multiple pore sizes and nucleoside loading options:

• 500 Å: for oligos <50 nt (e.g., ASO, siRNA)
• 1000 Å: for oligos <100 nt
• 2000 Å: for oligos <200 nt (e.g., gRNA)