1. Product Overview
Product Name: dG(dmf)-CPG (Co-Polymer Coated, HL-CPG)
Product Positioning:
Designed for the incorporation of unmodified dG at the 3′ end of oligonucleotides.
Suitable for the synthesis of challenging, long, and modified oligonucleotides.
Compared with traditional dG(dmf)-CPG, our dG(dmf)-CPG (Co-Polymer) features a new CPG solid support architecture (polystyrene + crosslinked CPG). In addition to high loading and high purity, this new support provides better compatibility with modified monomers and improved batch-to-batch stability.
Product Form: Powder
Product Color: White powder
Packaging: Glass bottle
2. What is dG(dmf)?
2.1 Chemical Identity of dG(dmf)
Full Name: 2′-Deoxyguanosine, N²-dimformyl protected
Molecular Formula: C11H15N5O5
Nucleoside Structure:
Base: Guanine (G)
Sugar: 2′-Deoxyribose
Protecting Group: N²-dimformyl (dmf) – protects the exocyclic amino group of G, prevents side reactions, improves coupling efficiency, and stabilizes DNA synthesis
2.3 Role of dG(dmf) in Oligonucleotide Synthesis
Serves as a 3′-terminal nucleoside for DNA oligonucleotide synthesis.
Positioned at the 3′ end of the solid support, enabling stable and efficient incorporation of guanine during solid-phase synthesis.
3. Application of dG(dmf)-CPG
dG(dmf)-CPG is used for the synthesis of G-rich DNA oligonucleotides, including antisense oligonucleotides (ASO), DNA probes, DNA primers, and gene-editing DNA templates.
| Application | Details | Typical Length | Notes / Features |
|---|---|---|---|
| ASO (Antisense Oligonucleotides) | DNA-like ASO drugs such as Spinraza® (nusinersen, Gapmer DNA), Mipomersen | 15–25 nt | G-rich regions prone to side reactions; dG(dmf)-CPG protects G to improve purity and yield |
| DNA Probes / Diagnostic Oligos | Used in PCR, qPCR, FISH, genetic testing | 20–60 nt | G-rich probes in long sequences benefit from dG(dmf)-CPG to enhance purity and coupling efficiency |
| DNA Primers | Used for PCR, qPCR, NGS library construction | 30–60 nt | G-rich primers require dG(dmf)-CPG as the 3′ solid support to improve synthesis stability |
| Gene Editing DNA Templates (non-RNA) | Used for in vitro PCR or modified DNA oligos | >100 nt | Long DNA sequences require dG(dmf)-CPG to improve coupling efficiency and synthesis stability |
4. Difference Between dG(dmf)-CPG vs dG(ibu)-CPG
| Feature | dG(iBu)-CPG | dG(dmf)-CPG |
|---|---|---|
| Protecting Group | N²-isobutyryl (iBu) | N²-dimethylformamidine (dmf) |
| Deprotection Conditions | Stronger acid required | Milder conditions, suitable for sensitive/highly modified sequences |
| Optimal Use | Standard DNA oligos, Gapmer ASO, primers, DNA probes | G-rich long-chain DNA, high-modification DNA, industrial-scale synthesis |
| Stepwise Yield | High | Higher, especially for long G-rich sequences |
| Crude Oligo Purity | High | Higher, fewer side reactions |
| Batch-to-Batch Consistency | Good | Excellent, suitable for GMP/industrial production |
| Applications | ASO 15–25 nt, DNA probes 20–60 nt, DNA primers 30–60 nt, gene-editing DNA templates >100 nt | Same as iBu, but performs better for long, G-rich or heavily modified DNA |
5. Why Using PoresynSolutions’ Co-Polymer Coated CPG?
Poresyn Solutions’ new co-polymer coated CPG (polystyrene–CPG crosslinked) significantly optimizes oligonucleotide production workflows. By using our support, the oligonucleotide output can be substantially increased while purification costs are reduced by over 30%. Our CPG enables GMP-scale oligonucleotide production, saving biotech companies time and labor. By improving FLP output, we help accelerate the development and publication of oligonucleotide drugs, making industrial-scale GMP production achievable.
| Category | Features / Details |
|---|---|
| Why Choose Poresyn dG(iBu)-CPG | Traditional dG(iBu)-CPG often faces low FLP output and <60% crude oligo purity. Our patented polystyrene + CPG crosslinked support addresses these issues, improving yield, purity, and stability for DNA oligo synthesis. |
| High-Loading HL-CPG Platform |
|
| Chemical & Mechanical Stability | Resistant to strong base, oxidation, and sulfurization conditions; suitable for prolonged automated synthesizer operation. |
| GMP & Scale-Up | Supports synthesis from µmol to mmol scale; suitable for CDMO/CMO scale-up; strong process transferability for industrial applications. |
| Cost & Time Efficiency | Reduces production time, labor costs, and purification expenses. |
| Quality Control – Raw Material | Produced strictly according to ISO standards. Key parameters (pore volume, pore size, surface area) controlled for batch consistency. Tested with BET nitrogen adsorption and laser particle size analyzers. |
| QC Testing | Post-production testing includes nucleoside loading, visual inspection, swelling assessment, and verification in DNA oligo synthesis to ensure reliability and stability. |
| Batch Traceability | Every batch is fully traceable from production to shipment, ensuring known batch histories for technical support and reliable GMP/industrial-scale applications. |
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