rG(DMF) CPG Co-Polymer Coated

rG(DMF)-CPG (Co-Polymer Coated)

What is rG(DMF)?

rG(DMF) stands for N2-Dimethylformamidine-guanosine,
a protected guanosine building block used in RNA solid-phase synthesis.

• r (ribo): RNA sugar (ribose).
• G (Guanosine): Guanine nucleobase.
• DMF (dimethylformamidine): Protecting group at the N2 position of guanine.

About the DMF Protecting Group

The N2 amino group of guanine is reactive during RNA synthesis.
The DMF protecting group prevents side reactions during coupling, oxidation, and sulfurization.

DMF protection is widely used in Fast RNA chemistry
and allows efficient post-synthesis deprotection to restore native riboguanosine.

• Reliable base protection
• Fast deprotection compatibility
• High coupling efficiency
• Accurate restoration of rG

Applications of rG(DMF)-CPG

• siRNA: Precise 3′ incorporation of riboguanosine.
• CRISPR gRNA / sgRNA: Ideal when 3′ terminal base is G.
• mRNA fragments: Defined 3′ ribo-G with modification compatibility.

For sequences requiring ribose 2′-hydroxyl protection, you can consider 2′-OAC CPG Co-Polymer Coated solution for improved stability and synthesis efficiency.

Benefits of Poresynsolutions‘ Co-Polymer Coated rG(DMF)-CPG in GMP-Scale RNA Production

Ultra-High Loading Capacity

The co-polymer coated rG(DMF)-CPG solid support is a member of poresynsolutions’ RNA modified CPG group, it delivers 3–6× higher loading capacity compared to conventional functionalized CPG supports, significantly increasing RNA oligonucleotide output per synthesis batch.

Typical loading levels:

• 500 Å: 200–240 μmol/g

• 1000 Å: 100–150 μmol/g

• 2000 Å: 40–80 μmol/g

This enhanced loading performance is particularly advantageous for long RNA synthesis (>100 nt) and large-scale GMP manufacturing, where productivity and cost efficiency are critical.

High Crude RNA Purity

The advanced co-polymer coating exhibits excellent resistance to:

• Acidic detritylation conditions

• Basic deprotection steps

• Organic solvents used during coupling cycles

As a result, crude RNA purity typically exceeds 80%, reducing downstream purification requirements and improving overall process efficiency in GMP production workflows.

Stable Physical Structure

Unlike traditional polystyrene-based resins that may swell in organic solvents, the rigid CPG core structure:

• Maintains dimensional stability

• Provides consistent flow characteristics

• Prevents bed compression during large-scale synthesis

This structural robustness is essential for reproducible RNA API manufacturing under GMP conditions.

No Silica Shedding Risk

The specialized co-polymer coating firmly stabilizes the CPG surface, minimizing:

• Silica particle shedding

• Column clogging

• Backpressure fluctuations

This ensures reliable synthesis of long and complex RNA oligonucleotides, including:

• siRNA

• CRISPR gRNA

• Long-chain mRNA fragments

Recommended Pore Size Selection

Proper pore size selection improves diffusion efficiency and synthesis yield.
Larger pores are recommended for long RNA strands, while smaller pores maximize loading for short oligos.

Protection Strategies and Support Selection

Nucleobase protection strategies are similar to those used in RNA phosphoramidites:

• Classic: Bz-A, Bz-C, iBu-dG
• UltraMILD: Pac-A, Ac-C, iPr-Pac-G
• Fast: Ac-C, Bz-A, DMF-G

However, key differences exist in 2’-OH protection and support characteristics:

Summary

Co-polymer coated rG(DMF)-CPG combines advantages of traditional functionalized CPGs and resin support, it delivers:

• 3–6× higher loading capacity
• >80% crude RNA purity
• Improved mechanical stability
• GMP-compatible large-scale RNA synthesis
• Long chain RNA synthesis