Poresyn Solutions - RNA Modified CPG Co-Polymer (HL-CPG) Manufacturer
Poresyn Solutions’ RNA Modified CPG co-polymer offer:
Up to 160 μmol/g loading with high purity, optimized for synthesizing RNA over 100 nt.
Small MOQ
We support small MOQs, starting from 10 g, for the Universe linker type.
Tailored Solutions
No matter what kind of linker you need, we can fulfill unique requests.
Free Sample For Test
Providing small sample of our HL-CPG with Unylinker for testing without charge
Free Delivery
Free air shipping, typically, the things arrive within seven days.
RNA Modified CPG (Co-Polymer)
Why use co-polymer RNA CPG
- Super high loading: Usually three to six times higher than the corresponding functionalized CPGs.
- High crude purity: When compared to conventional CPGs, we attain a crude oligo purity that is around 30% higher.
- Proven reliability: Our HL-CPG has been shown to produce reliable results for RNA synthesis up to 200 nt.
- Long and complicated gRNA synthesis: Our HL-CPG can consistently synthesize RNA longer than 100 nt, producing A260 OD values that are many times greater than those of traditional CPGs, even for complex gRNAs.
- GMP-compliant safety: eliminates the risk of CPG fragments obstructing synthesis equipment, is chemically inert, doesn't swell, and doesn't require a certain loading density.
2000A Co-Polymer Coated CPG
Why is it difficult to synthesize RNA that is more than 100 nt long?
Because RNA monomers are quite large, the CPG’s pore capacity and homogeneity are crucial when the chain length surpasses 100nt; otherwise, coupling effectiveness may drastically decline. Furthermore, RNA is prone to side reactions both inside and between chains due to its complex and irregular structure. Long RNA strands are more prone to deterioration or contaminant production during deprotection, oxidation, sulfurization, and other synthesis processes because of their chemical sensitivity. Furthermore, typical CPGs have a restricted loading capacity, which results in low FLP output that cannot satisfy industrial-scale production demands. Additionally, as the length of the RNA chain rises, the overall yield falls.
Why the 2000A Co-Polymer Coated CPG from Poresyn Solutions is perfect
For complicated long-chain RNA？
Our 2000A Co-Polymer Coated CPG was created especially. We offer highly uniform CPG pore diameters with nanoscale resin coating thickness that improves chemical reactivity while having no impact on pore size. Our HL-CPG solid support substantially higher FLP output, with A260 OD values several times higher than ordinary CPGs, thanks to its 3–6× larger loading capacity and superior coupling efficiency.
Significantly reduce the oligo purification cost
During synthesis and subsequent treatment, our co-polymer coated CPG remains inert to chemical reagents and solvents. As a result, there are less contaminants. Furthermore, our coupling efficiency is higher than that of traditional CPGs, usually exceeding 99%, guaranteeing a higher percentage of FLP crude product. Additionally, our product shows no signs of swelling or silica contamination, which further lowers impurities and guarantees manufacturing safety. Our overall crude purity is more than 30% greater than that of conventional CPGs, which results in substantial cost savings for biotech companies.
What is RNA Modified CPG Co-Polymer
RNA-modified CPG ： A solid-phase carrier (Controlled Pore Glass, CPG) that has been preloaded with an initial RNA nucleotide for RNA synthesis—typically a protected RNA monomer like A, C, G, or U, or modified monomers like 2′-O-Me or 2′-F. It is where solid-phase RNA synthesis begins.
Co-Polymer coated CPG RNA modified
The new patented CPG based solid support, also known as hyper-link CPG, offers high loading capacity, high coupling efficiency, high purity, and excellent chemical reactivity by cross-linking CPG with a specialized resin material. It allows for the stable synthesis of complex RNA up to 200 nucleotides in length.
HL-CPG
Our RNA Modified CPG (Co-Polymer)
Following are the principal monomer types linked to our co-polymer coated CPG solid support for RNA synthesis.
OUR PRODUCT
Superior Advantages of our HL-CPG (Co-Polymer Coated CPG Solid Support)
By utilizing our HL-CPG, users typically achieve a 30–70% reduction in cost of goods (COG) compared with conventional functionalized CPG, thanks to its high loading capacity, superior purity, and overall process efficiency
High Loading
3–6× higher loading than traditional CPG supports — e.g., our 2000Å HL-CPG achieves 160 μmol/g
High Purity
Delivers >80% oligo purity, owing to strong chemical resistance to reagents and solvents
No Swelling
Unlike traditional polystyrene supports, our HL-CPG remains stable and does not swell under reaction or heating conditions, ensuring consistent synthesis performance
High surface reactivity
The ps layer provides high surface reactivity, resulting in outstanding synthesis performance even when no LCAA is coupled.
No Silica Contamination
No glass particles are generated during ammonolysis, thanks to the PS surface crosslinked with the CPG core structure, ensuring cleaner synthesis and easier purification.
Fully Compatible Setup
Fully compatible with existing CPG-based synthesis setups — no equipment modification required
Why RNA Synthesis is Generally More Challenging than DNA Synthesis
Due to the presence of a 2′-OH group on the ribose, compared with DNA, RNA is more likely to have coupling failures due to its susceptibility to strong acids and bases as well as intramolecular and intermolecular transesterification side reactions (2′→3′ transfer). Furthermore, coupling efficiency is reduced by the large size and strong protection of RNA phosphoramidite monomers. Chain cleavage can be easily caused by the more complicated deprotection procedures. Steric hindrance builds up for lengthy RNA sequences (>100 nt), which dramatically reduces the success rate and usually results in a very poor FLP yield.
These issues are explicitly addressed by Poresyn Solutions’ RNA-modified HL-CPG, which opens the door to GMP-scale industrial production of RNA oligonucleotides while simplifying, improving, and lowering the cost of RNA synthesis.
Which RNA nucleoside monomers are supported on your HL-CPG?
rA (Bz), rC (Ac), rC (Bz), rG (iBu), rG (dmf), rU;
2′-OMe rA (Bz), 2′-OMe rC (Ac), 2′-OMe rC (Bz), 2′-OMe rG (iBu), 2′-OMe rG (dmf), 2′-OMe rU, 2′-O-A, tA (Ac), 2′-OAc, etc.
Have you tested your HL-CPG on the actual RNA synthesis performance?
Yes, we have conducted multiple tests, for example, synthesizing a 170-nt gRNA, achieving excellent results with ultra-high loading levels and high FLP output as expected.
Can your RNA modified HL-CPG synthesize long-chain gRNA, such as those over 100 nt?
Yes, our HyperLink CPG solid support is primarily designed for long-chain oligonucleotide synthesis, and it performs excellently for oligos up to 300 nt.
Can your co-polymer coated CPG be integrated with a cell delivery system?
Actually, we can install cell delivery like GALNAC much more easily and effectively than with equivalent functionalized CPG because the ps layer is covered with the core structure of controlled pore glass.
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Related Product
Custom modifications of our co-polymer coated controlled pore glass, such as DNA/RNA modified HL-CPG, 2′-O-Methyl modified, reverse functionalized, LNA modified, 2′-F modified, and others, are typically available upon request.
DNA Modified CPG Co-Polymer
2'-O-Methyl Modified CPG Co-Polymer
Reverse Functionalized CPG Co-Polymer
LNA Modified CPG Co-Polymer
2'-F Modified CPG Co-Polymer
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Test the power of our Super Solid Support.
Would you like to test our next-generation co-polymer coated CPG? Get in touch with us right now; we offer a free sample of our HL-CPG with unylinker for rapid testing.