Custom Long DNA Oligo Synthesis Service
Achieving high-fidelity ultra-long oligonucleotides (100–300+ nt) for demanding genomics applications.
Lengths up to 300+ bases
Enabling complex gene assembly and CRISPR research with industry-leading sequence lengths.
>99% Coupling Efficiency
Superior chemical accuracy ensures higher yields of full-length, high-quality DNA.
100% MS & PAGE Purified
Every oligo is verified by Mass Spec and PAGE to guarantee 100% sequence integrity.
Powered by Hyper-Link CPG
Proprietary support matrix optimizes synthesis environment for complex and dense sequences.
Next-Generation Long Oligo Synthesis
Empower your advanced genomics and therapeutic applications with Poresyn’s Custom Long DNA Oligo Synthesis. Built upon our proprietary non-swelling PS+CPG Crosslinked architecture, our service overcomes traditional bottlenecks—such as low yields and pipeline clogging—to seamlessly scale your complex sequences from R&D directly to GMP production. Delivered ready-to-use, our high-fidelity oligos provide a reliable, high-purity foundation for your most critical CRISPR, cloning, and gene assembly workflows
Seamless Scale-Up for Extended DNA Sequences
Synthesizing extended DNA sequences (100–300+ nt) inherently amplifies purity and yield bottlenecks, especially when transitioning from R&D to GMP production. Our custom long oligo service is engineered to eliminate this gap. Because our Hyper-Link solid support prevents swelling and accommodates high-density synthesis, it effectively minimizes the cumulative truncation errors typical of long-chain assembly. We guarantee reproducible scaling for your most critical workflows—such as ultra-long CRISPR HDR templates and synthetic gene assemblies—delivering consistent full-length purity whether you need 100 nmol or large-scale GMP batches.
Long DNA Oligonucleotide Synthesis Specifications
High-fidelity synthesis from 100 to 300+ nucleotides
| Length | Available Scales | Purification | Deliverables |
|---|---|---|---|
| 100–150 nt |
| PAGE (Recommended) or HPLC |
|
| 151–200 nt |
| PAGE Required |
|
| 201–300+ nt |
| PAGE Required |
|
The Technology Edge: Why Choose Poresyn?
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Overcoming Traditional Limits
Powered by Co-Polymer Coated CPG
Long DNA Oligonucleotide Synthesis Applications (100–300+ nt)
Our long DNA oligonucleotide synthesis platform supports a wide range of applications, covering sequences from 100 to over 300 nucleotides. We provide high-purity, PAGE-validated oligos for use in gene synthesis, PCR, CRISPR genome editing, next-generation sequencing (NGS), molecular diagnostics, and therapeutic development.
This section highlights the major types of long DNA oligos we routinely manufacture, including gene fragments, long PCR primers, CRISPR donor templates, DNA aptamers, hybridization probes, antisense oligonucleotides, oligo libraries, and modified DNA sequences. With scalable production from research quantities (nmol–µmol) to multi-gram and industrial-scale manufacturing, we support projects from early discovery to clinical and commercial applications.
Rigorous Quality Assurance & Regulatory Compliance
At Poresyn, every custom long DNA oligo is synthesized under a rigorous ISO 9001-certified quality management system. Our facilities operate at GLP laboratory standards and are fully equipped for GMP-grade manufacturing, ensuring a seamless transition for clinical-stage projects. We employ advanced analytical platforms—including ESI-MS, MALDI-TOF, and high-resolution PAGE/CE—to verify 100% sequence identity and full-length purity. We back our industry-leading fidelity with a 30-day satisfaction guarantee, providing free remakes or credits to ensure your complete confidence in every sequence.
Long Oligonucleotide Synthesis Solutiosn
Beyond standard sequences, we provide a comprehensive suite of specialized long-mer services tailored to your project’s scale. From high-fidelity HDR templates for CRISPR research to multi-gram industrial supply of complex DNA and modified RNA, our platform ensures seamless transition from benchtop discovery to large-scale manufacturing.
Long RNA Oligos & Modified RNA
Long ssDNA Megamers (HDR Templates)
Complex & GC-Rich Long Oligos
IVT Templates for mRNA Synthesis
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More Questions You May Have
How do you handle sequence secondary structures that typically interfere with synthesis?
While many providers reject sequences with high GC content or complex hairpins, our Hyper-Link CPG technology is specifically designed to minimize steric hindrance. We utilize proprietary thermal-stable phosphoramidites and optimized deprotection protocols to “relax” secondary structures during the elongation phase, ensuring that even highly structured sequences reach their full intended length with high crude purity.
Can I request custom aliquoting or specific buffer formulations for industrial batches?
Absolutely. We understand that industrial-scale projects (mg to 100g+) often require direct integration into automated pipelines. We offer flexible downstream processing, including custom concentration normalization, specific buffer dissolution (e.g., TE, DNase-free water), and barcoded secondary packaging to match your facility’s internal SOPs.
What is the typical turnaround time (TAT) for a 300nt PAGE-purified oligo?
For long-mers (100–300nt), the synthesis and stringent PAGE purification process typically takes 10–15 business days, depending on the complexity and scale. We prioritize quality over speed for these sensitive lengths to ensure that the delivered product meets our >90% purity benchmark. Expedited shipping options are available for urgent R&D milestones.
Do you provide batch-to-batch consistency reports for long-term clinical projects?
Yes. For clients transitioning from R&D to GMP-grade production, we provide Batch Release Records (BRR) and longitudinal stability data upon request. Our ISO 9001-certified management system ensures that the raw materials (including our proprietary CPG) and synthesis parameters remain constant, providing the high reproducibility required for regulatory filings.
Is it possible to incorporate internal modifications (e.g., Phosphorothioate) at specific positions in a 250nt sequence?
Yes, our platform supports site-specific internal modifications. Unlike some high-throughput providers that only offer 5′ or 3′ modifications, we can incorporate Phosphorothioate (PS) bonds, methylated bases, or fluorophores at designated internal positions even in ultra-long sequences, thanks to the high coupling efficiency of our co-polymer coated support.
What happens if my sequence fails to meet the purity requirements during QC?
At Poresyn, we operate on a success-based model. Every long oligo undergoes mandatory LC-MS and PAGE verification. If a sequence fails to meet our rigorous Certificate of Analysis (CoA) standards during our internal QC, we will automatically re-synthesize it using alternative chemistry protocols at no additional cost to you. We only ship products that meet 100% of the promised specifications.
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Ready to Start Your Long DNA Project?
Experience industry-leading coupling efficiency and guaranteed purity for your custom long oligos. Whether it’s 100 nmol or 1000 grams, Poresyn delivers high-fidelity DNA tailored to your exact specifications. Get an instant quote and start your synthesis journey now.