Poresyn Solutions Hyper Link Reverse CPG
3′-DMT Functionalized HL-CPG (Reverse)
With superior high loading and exceptional UV purity, we help maximize FLP output and significantly reduce purification costs, perfectly supporting GMP-scale oligonucleotide production.
Small MOQ
We support small MOQs, starting from 10 g, for the Universe linker type.
Tailored Solutions
No matter what kind of linker you need, we can fulfill unique requests.
Free Sample For Test
Providing small sample of our HL-CPG with Unylinker for testing without charge
Free Delivery
Free air shipping, typically, the things arrive within seven days.
New Reverse CPG (Co-Polymer) Solid Support for Oligonucleotide
GMP-Oriented Manufacturing-Key Benefits
- Super High Loading Capacity: The loading capacity is three to six times greater than that of corresponding functionalized CPGs, increasing the overall oligo yield.
- High Oligo Purity: 30 percent greater UV purity than conventional CPGs will achieve, lowering the cost of downstream purification and enabling GMP-scale manufacturing.
- Chemical & Solvent Stability: Our solid support resists swelling and dissolution, maintaining stability under harsh reagents and temperature conditions
- Uniform Particle & Pore Size: Uniform CPG particles ensure even synthesis reactions, and stable pore sizes prevent clogging and maintain high coupling efficiency even for long-chain oligos.
- Platform Compatibility: Compatible with a variety of linkers and modifications, including LNA, 2'-OMe, and 2'-F, adapt to current synthesis equipment and procedures without alteration.
- Special Functionality: Allows direct installation of modified nucleotides or delivery systems (e.g., GalNAc, cholesterol), reducing additional synthesis steps and purification needs.
Effective and Stable FLP Output
Moreover, the final oligo yield is significantly improved by our HL-CPG, and the advantages become more noticeable for longer sequences (eg., 100nt+).
This process guarantees that the CPG material has consistent porosity and particle sizes while preserving outstanding batch-to-batch uniformity.
As a result, the co-polymer coated CPG can produce crude oligo A260 OD values several times higher than standard CPGs due to its high loading capacity and improved purity.
For instance, our 2000 ¹ HL-CPG can obtain synthesis OD values up to 5000 for oligos over 100 nt.
Significant Purification Cost Savings
In contrast, traditional CPGs often produce oligos with lower purity due to issues such as uneven particle size, insufficient glass strength, or limited chemical stability, which can lead to dissolution of silica particles or other impurities during deprotection or other chemical reactions. Poresyn Solutions’ co-polymer coated CPG, with its unique polystyrene + CPG crosslinked structure, ensures high-purity oligos. The solid support is inert to chemical reagents and solvents during synthesis and subsequent treatments.
Its stable and uniform physical structure allows consistent delivery of oligos with purities over 30% higher than conventional CPGs, helping biotech companies save substantial downstream purification costs.
What is Reverse CPG Solid Support
Reverse CPG is characterized by having its linker connection point oriented in the opposite direction compared with normal CPG.
- Normal CPG: The nucleoside like A, C, G, T is attached to the CPG solid support through its 3′-OH, and the synthesis sequence proceeds in the 3’→5’ direction.
- Reverse CPG: The nucleoside is connected through a special 5′-linker, causing the synthesis sequence to be changed to 5’→3’.
Reverse CPG Application
Reverse CPG is mainly used for:
Synthesizing oligos with special 5′-end structures
Modifications that must be started from the 5′-end
Specific architecture needs in RNA, LNA, or other modified oligonucleotides
Typical applications include constructing special structural motifs in siRNA, ASO, and aptamers.
Co-Polymer Coated Reverse CPG
3′-DMT Protected Nucleoside-Linked Reverse CPG Co-Polymer Coated
Here are the main monomer types featuring 3′-DMT protected nucleosides linked to our co-polymer coated CPG solid support, designed for DNA synthesis.
OUR PRODUCT
Superior Advantages of our HL-CPG (Co-Polymer Coated CPG Solid Support)
By utilizing our HL-CPG, users typically achieve a 30–70% reduction in cost of goods (COG) compared with conventional functionalized CPG, thanks to its high loading capacity, superior purity, and overall process efficiency
High Loading
3–6× higher loading than traditional CPG supports — e.g., our 2000Å HL-CPG achieves 160 μmol/g
High Purity
Delivers >80% oligo purity, owing to strong chemical resistance to reagents and solvents
No Swelling
Unlike traditional polystyrene supports, our HL-CPG remains stable and does not swell under reaction or heating conditions, ensuring consistent synthesis performance
High surface reactivity
The ps layer provides high surface reactivity, resulting in outstanding synthesis performance even when no LCAA is coupled.
No Silica Contamination
No glass particles are generated during ammonolysis, thanks to the PS surface crosslinked with the CPG core structure, ensuring cleaner synthesis and easier purification.
Fully Compatible Setup
Fully compatible with existing CPG-based synthesis setups — no equipment modification required
Why Poresyn Solutions HL-CPG Solves GMP-Scale Oligonucleotide Manufacturing Challenges
Considering that Reverse CPG is primarily used for oligonucleotides’ length less than 100 nt, our 500 Å and 1000 Å HL-CPG is optimized for this length range. Our PS+CPG cross-linked architecture offers better performance during actual synthesis, increased linker stability, and a stronger affinity for attaching unique nucleotides than ordinary CPG.
By using Poresyn Solutions’ co-polymer coated CPG, the final A260 OD output can be 3–5× larger than that of corresponding functionalized CPG.
This is due to our extraordinarily high loading capacity (up to 240 µmol/g for 500 Å HL-CPG, 180 µmol/g for 1000 Å HL-CPG) and improved coupling efficiency.
The co-polymer coated CPG (HL-CPG) perfectly avoids swelling while maintaining a high loading capacity, in contrast to polystyrene that has a tendency to swell during synthesis and result in production safety issues. Our unique PS+CPG crosslinked solution has a significant benefit for GMP-scale automated synthesis because of its structural stability.
Overall, the co-polymer coated HL-CPG solid support is the most reliable and efficient solution for industrial oligonucleotide synthesis.
High Performance Reverse CPG for 5′-End Oligo Synthesis
Futhermore, clean and efficient reverse-direction synthesis (5′→3′) without side reactions or orientation errors is made easy by our Reverse Co-Polymer Coated HL-CPG, which guarantees precise and stable 5′-end attachment of the starting nucleotide.
High structural integrity is ensured throughout the whole workflow by using to the 5′-linker chemistry on our solid support, which is designed for remarkable stability under all synthesis conditions, including strong acids, strong bases, oxidation, sulfurization, and deprotection steps.
High Adapabilities of Poresyn Solutions' Reverse CPG Co-Polymer
Additionally, our Reverse HL-CPG works perfectly with a variety of sophisticated monomers and functional groups.
These include LNA, RNA monomers 2′-OMe and 2′-F, reporter tags, special functional groups, and complicated 5′-end alterations.
Poresyn Solutions’ Reverse HL-CPG is a great solution for high-value oligos with particular 5′-end structures because of excellent orientation control, chemical durability, and versatile compatibility.
Can your HL-CPG synthesize long-chain gRNA, such as those over 100 nt?
Yes, our HyperLink CPG solid support is primarily designed for long-chain oligonucleotide synthesis, and it performs excellently for oligos up to 300 nt.
Can you add C6 or C3 LCAA on your HL-CPG?
Sure, we can connect C3 or C6 LCAA to our co-polymer coated CPG to further enhance the reactive activity during oligonucleotide synthesizing process.
What pore sizes do you typically offer for your reverse CPG?
Since reverse CPG is typically used for oligonucleotides < 100 nt, we offer 500 Å and 1000 Å of our HL-CPG as our standard options. For special application, we can also provide custom pore sizes upon customer’s request.
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Related Product
Custom modifications of our co-polymer coated controlled pore glass, such as DNA/RNA modified HL-CPG, 2′-O-Methyl modified, reverse functionalized, LNA modified, 2′-F modified, and others, are typically available upon request.
RNA Modified CPG Co-Polymer
DNA Modified CPG Co-Polymer
2'-O-Methl Modified CPG Co-Polymer
LNA Modified CPG Co-Polymer
2'-F Modified CPG Co-Polymer
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Test the power of our Super Solid Support - Reverse CPG Co-Polymer
Would you like to test our next-generation co-polymer coated CPG? Get in touch with us right now; we offer a free sample of our HL-CPG with unylinker for rapid testing.