Custom Extra LONG RNA SYNTHESIS Service
Achieving high-fidelity, ultra-long RNA oligonucleotides (100–300+ nt) with our advanced LONG RNA SYNTHESIS platform for demanding therapeutic and screening applications.
Lengths up to 300+ bases
Overcoming traditional length barriers in LONG RNA SYNTHESIS to enable complex guide RNA assembly, long-mer RNAi, and advanced structural biology research.
>99% Stepwise Coupling Efficiency
Superior chemical accuracy engineered specifically to maximize full-length product recovery during the LONG RNA SYNTHESIS process.
100% MS & PAGE Purified
Every custom sequence from our LONG RNA SYNTHESIS service is strictly verified by Mass Spec and high-resolution PAGE to guarantee complete sequence integrity.
Powered by Hyper-Link CPG
Our proprietary co-polymer coated solid support optimizes the chemical microenvironment for dense, complex, and highly structured LONG RNA SYNTHESIS workflows.
Next-Generation Long-Mer Manufacturing
Empower your advanced nucleic acid therapeutics, CRISPR screening, and functional RNA research with Poresyn’s premium LONG RNA SYNTHESIS solutions. Built upon our proprietary non-swelling PS+CPG Crosslinked architecture, our specialized LONG RNA SYNTHESIS platform overcomes traditional manufacturing bottlenecks—such as cumulative truncation errors, low crude yields, and severe steric hindrance.
We engineer our platform specifically for inherent sequence complexity, allowing us to support highly challenging long RNA constructs with careful, precise attention to your specific sequence composition, total length, and customized synthesis strategy.
Delivered ready-to-use, our high-fidelity long-mer RNA oligos provide a reliable, high-purity foundation for your most critical workflows. To guarantee this uncompromising purity, each RNA oligonucleotide undergoes rigorous quality control using industry-standard techniques such as PAGE, HPLC, and/or mass spectrometry (MS). We seamlessly scale your complex sequences directly from benchtop R&D to large-scale GMP production.
Seamless Scale-Up for Extended RNA Sequences
Synthesizing extended RNA sequences (100–300+ nt) inherently amplifies purification and yield bottlenecks due to the delicate nature of RNA chemistry and the efficiency drops typical of long-chain assembly. Our custom LONG RNA SYNTHESIS service is specifically engineered to eliminate this gap, especially when moving into structurally demanding designs.
Because we build every process for sequence complexity, we support challenging long RNA constructs with careful attention to sequence composition, length, and synthesis strategy. Our Hyper-Link solid support prevents swelling and accommodates high-density synthesis without structural collapse, delivering up to 3x higher loading capacity compared to traditional CPG supports. This specialized microenvironment minimizes cumulative truncation errors (n-1, n-2) and achieves high crude purity during chemical elongation.
Rigorous Quality Control & Industrial-Grade Assurance
To ensure complete peace of mind during deployment and scale-up, every custom sequence manufactured via our LONG RNA SYNTHESIS production line undergoes multi-tiered, rigorous quality control using high-resolution analytical platforms. We guarantee reproducible scaling for your critical workflows—delivering consistent full-length purity (FLP) whether you need nanomolar research quantities or multi-gram industrial batches.
Our specialized facility operates under a strict, ISO 9001:2015 certified quality management system, balancing GLP laboratory precision with full equipment capabilities for GMP-grade raw material scaling to secure a seamless regulatory transition for clinical-stage pipelines.
Furthermore, we extend our quality assurance into protective downstream handling. To safeguard delicate chemical stability and eliminate any risk of ribonuclease degradation, final long-mer RNA products are sealed in certified RNase-free and DNase-free vials backfilled with high-purity Nitrogen ($N_2$) gas environments, with large-scale industrial batches dispatched via verified cold-chain logistics. We back our industry-leading fidelity with a 30-day satisfaction guarantee, providing free remakes or credits to ensure total confidence in your custom sequences.
To verify total sequence fidelity, 100% sequence identity, exact molecular weight, and full-length uniformity, we systematically employ advanced characterization techniques:
Mass Spectrometry (ESI-MS & MALDI-TOF): Securing absolute verification of mass accuracy and sequence identity.
Chromatography & Electrophoresis (HPLC & High-Resolution PAGE/CE): Delivering definitive profiling of purity gradients and full-length product ratios.
Extra Long RNA Oligonucleotide Synthesis Specifications
High-fidelity LONG RNA SYNTHESIS from 100 to 300+ nucleotides
RNA Type Matrix: Comprehensive support for custom long RNA including mRNA fragments, tRNA, lncRNA, and advanced guide RNA-related constructs.
Dual Synthesis Methods: Solid-phase chemical synthesis optimized for sequences up to 300+ nt. For extended lengths or specific project designs, high-efficiency In Vitro Transcription (IVT) options are fully integrated.
Form & Structure Flexibilities: Available in extended single-stranded RNA (ssRNA) and double-stranded RNA (dsRNA) constructs tailored for cutting-edge research applications.
Extensive Modification Options: Full compatibility with base, sugar, and backbone modifications, alongside premium functional labeling (e.g., fluorescent dyes, biotin, and specialized click chemistry).
Industrial-Grade Packaging: Delivered using specialized RNaseSAFE™ packaging in strictly validated RNase- and DNase-free tubes/vials, complete with high-purity Nitrogen ($N_2$) backfilling for maximum stability.
Broad Downstream Applications: Engineered directly for therapeutic research, CRISPR gene-editing workflows, assay development, structural biology, and synthetic biology.
| Length | Available Scales & Yield | Purification Options | Quality Control & Deliverables |
|---|---|---|---|
| 100–150 nt |
| PAGE (Highly Recommended), HPLC, Cartridge, or Desalting (Based on sequence & application) | QC: Rigorous verification via PAGE, HPLC, and/or Mass Spectrometry (MS). Deliverables: Lyophilized powder in RNaseSAFE™ vials, LC-MS MW confirmation, Purity analysis profile, and CoA. |
| 151–200 nt |
| PAGE Required (HPLC optional based on sequence complexity) | QC: Full-spectrum PAGE, HPLC, and mass spectrometry (MS) characterization. Deliverables: Lyophilized powder in RNaseSAFE™ vials, LC-MS MW confirmation, PAGE purity analysis, and CoA. |
| 201–300+ nt |
| PAGE Required to ensure maximum sequence fidelity and length uniformity | QC: Ultra-high resolution PAGE validation and comprehensive MS sequence mapping. Deliverables: Lyophilized powder in RNaseSAFE™ vials, LC-MS MW confirmation, high-resolution PAGE purity report, and CoA. |
The Poresyn Advantage: Unlocking the Future of Nucleic Acid Engineering
Overcoming the Barriers of Sequence Complexity
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Long-Mer Application Focus Areas (100–300+ nt)
PoresynTechnologies advanced LONG RNA SYNTHESIS chemistry supports a wide array of research and therapeutic applications. We provide high-purity, PAGE-validated RNA oligos spanning from 100 to over 300 nucleotides.
With production scales seamlessly transitioning from research quantities to multi-gram industrial manufacturing, we support your project from early-stage screening to clinical development.
Advanced RNA Modifications for Functional Enhancement
Chemical modifications can be seamlessly incorporated into extended sequences through our premium LONG RNA SYNTHESIS platform to dramatically improve serum stability, reduce immunogenicity, and enable specialized downstream applications. Every modification strategy is carefully selected and assessed based on your specific sequence design, synthesis feasibility, and intended application.
Base Modifications
Modify nucleobases (such as pseudouridine, 5-methylcytidine, or N6-methyladenosine) to support advanced structure-function studies, improve target recognition, or enhance cellular translation performance in innovative RNA tool development.
Sugar Modifications
Incorporate high-performance sugar ring modifications, including 2′-O-Methyl (2′-OMe), 2′-Fluoro (2′-F), and 2′-O-Methoxyethyl (2′-MOE), directly into extra-long constructs. These modifications provide exceptional nuclease resistance, shielding your long-mer RNA from rapid degradation.
Backbone Modifications
Modify the phosphate backbone using Phosphorothioate (PS) bonds to significantly enhance chemical durability against cellular nucleases and precisely tune biological interactions within advanced therapeutic workflows.
Precision Labeling
Add premium functional labels—including a broad range of fluorescent dyes, biotin, spacers, or click chemistry handles—at designated internal or terminal positions for high-sensitivity detection, molecular tracking, and custom assay development.
Targeted Bioconjugation
Optimize cellular delivery and tissue targeting. Our platform supports the solid-phase bioconjugation of advanced delivery vehicles, including GalNAc clusters, cholesterol, and specialized lipids, directly onto the 5′ or 3′ termini of highly complex, long RNA sequences.
Multi-Site Custom Strategies
Combine multiple modification types within a single, highly complex RNA construct. By pairing our proprietary Hyper-Link CPG with flexible coupling chemistry, we overcome severe steric hindrance to deliver tailored, multi-site modified long-mers that meet specific next-generation application needs.
Strategic Design Considerations
Successful modification selection depends heavily on overall sequence length, secondary structure impacts, and synthesis compatibility. Because long-chain modification inherently alters folding dynamics, our technical engineering team provides comprehensive project reviews to assist you in designing the most robust and stable synthesis strategy for your advanced research.
Terminal Architecture Engineering
Enhance translational efficiency and prevent exonuclease degradation by incorporating custom terminal structures into your LONG RNA SYNTHESIS projects. We offer precise engineering of 5′ and 3′ ends, including the integration of specialized chemical cap analogs and synthetic poly(A) tail variants. These modifications mimic natural mRNA architectures, significantly boosting stability and performance in cell-free translation assays and functional screening workflows.
Customization-Focused Platform
Every breakthrough workflow demands a precise foundation. Our infrastructure is completely customization-focused, allowing you to configure your LONG RNA SYNTHESIS parameters flawlessly from start to finish. You define the exact architecture your project requires:
Sequence Length & Scale: Seamless scalability from nanomolar screening arrays up to multi-gram industrial output.
Modification Strategy: Full integration of base, sugar, and backbone variations, along with functional labeling and terminal delivery conjugations.
Purification & Analytical Requirements: Tailored downstream processing utilizing Desalting, Cartridge, HPLC, or high-resolution PAGE, backed by rigorous quality control validation (MS, HPLC, and/or PAGE) to secure 100% sequence fidelity.
Specialized Long-Mer RNA synthesis Solutions
Beyond custom single-strand RNA synthesis, we provide a comprehensive suite of specialized integration services tailored to your project’s unique scaling requirements:
Long ssDNA Megamers (HDR Templates)
Complex & GC-Rich Long Oligos
IVT Templates for mRNA Synthesis
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More Questions You May Have
What is the maximum sequence length supported by your LONG RNA SYNTHESIS service?
Our advanced solid-phase chemistry platform supports custom LONG RNA SYNTHESIS for single-guide RNA, aptamers, and structured constructs up to 300+ nucleotides (nt) in length. For sequences exceeding this range, we also offer high-efficiency In Vitro Transcription (IVT) alternatives depending on your project requirements.
Can modified RNA be requested for extra-long sequences?
Yes. We offer comprehensive modification options including base, sugar, and backbone alterations (such as 2′-OMe, 2′-F, and Phosphorothioate bonds) as well as terminally conjugated delivery vehicles (GalNAc, cholesterol, and lipids). Modification feasibility is systematically evaluated during our initial technical project review based on sequence composition and your intended application.
What information should I provide to obtain a custom LONG RNA SYNTHESIS quote?
To receive an accurate and timely quote, please provide your exact RNA sequence (5′ to 3′), target length, desired yield scale (nmol to gram/kilogram quantities), specific modification requirements, purification preferences (such as PAGE or HPLC), and any relevant downstream application notes.
Do you have real-world case studies using your co-polymer coated CPG for LONG RNA SYNTHESIS over 100+ nt?
Yes. We have a proven track record of overcoming severe sequence complexity hurdles. We have successfully synthesized 175 nt extra-long RNA molecules with high coupling efficiency and structural fidelity by utilizing our proprietary Hyper-Link co-polymer coated CPG solid support matrix.
Can you provide specific technical parameters such as bead size, loading capacity, and pore size?
Yes. Full material specifications—including exact pore size selections (500A, 1000A, or 2000A), optimized high loading capacity ranges, and particle size metrics—are thoroughly documented and provided via a formal Certificate of Analysis (CoA) for your review once the manufacturing batch is completed.
How does your Hyper-Link CPG technology reduce downstream purification costs in LONG RNA SYNTHESIS?
Traditional supports often suffer from low loading capacities and high truncation rates ($n-1, n-2$) when synthesizing extended chains. Our proprietary co-polymer coated CPG delivers 3x higher loading capacity and maintains a non-swelling environment under high pressure. This results in significantly higher crude purity and full-length product (FLP) yield, which directly cuts downstream purification costs by over 30%.
What quality control (QC) methodologies are used to verify the integrity of long-mer RNA?
Every single oligonucleotide manufactured through our platform undergoes rigorous, multi-tiered quality control validation. We utilize high-resolution Polyacrylamide Gel Electrophoresis (PAGE), High-Performance Liquid Chromatography (HPLC), and Mass Spectrometry (MS) to verify exact molecular weight, sequence identity, and 100% full-length purity before delivery.
Are your custom LONG RNA SYNTHESIS products compatible with industrial scale-up platforms?
Absolutely. Because our proprietary crosslinked CPG matrix features zero swelling and exceptional physical rigidity, it eliminates bed compaction and fluidic clogging issues. The material is fully optimized for a smooth, reproducible transition from micro-scale laboratory R&D directly to large-scale automated platforms like the AKTA OligoPilot for GMP-grade manufacturing.
How are sensitive long-mer RNA oligos protected during packaging and shipping?
To eliminate the risk of ribonuclease degradation and oxidative damage, all extra-long RNA oligos are enclosed in specialized packaging using strictly validated RNase- and DNase-free tubes or vials. The containers are backfilled with high-purity Nitrogen (N2) gas environments, and large-scale bulk shipments are dispatched via qualified cold-chain logistics.
Do you support the synthesis of highly structured or GC-rich long RNA elements?
Yes. Our platform is specifically engineered for high sequence complexity. Repetitive regions, hairpins, riboswitches, and extreme GC-content often cause standard synthesis methods to fail. By optimizing the thermodynamic environment and utilizing our high-density Hyper-Link CPG, we regularly deliver complex and structured RNA constructs that other providers deem impossible.
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Experience industry-leading coupling efficiency and guaranteed purity for your custom long oligos. Whether it’s 100 nmol or 1000 grams, Poresyn delivers high-fidelity RNA tailored to your exact specifications. Get an instant quote and start your synthesis journey now.